Chromogenic System for Measuring Hydrogen Peroxide: The Enzymatic Uric Acid Assay

Chromogenic system for measuring hydrogen peroxide: the enzymatic uric acid assay.

Featured Article: Fossati P, Prencipe L, Berti G. Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clin Chem 1980;26:227–31. Our 1980 report in Clinical Chemistry described an improved chromogenic detection system that, coupled with the enzyme oxidation of uric acid, led to a direct method for assaying ...

Optimized enzymatic colorimetric assay for determination of hydrogen peroxide (H2O2) scavenging activity of plant extracts

The classical method to determine hydrogen peroxide (H2O2) scavenging activity of plant extracts is evaluated by measuring the disappearance of H2O2 at a wavelength of 230 nm. Since this method suffers from the interference of phenolics having strong absorption in the UV region, a simple and rapid colorimetric assay was developed where plant extracts are introduced to H2O2, phenol and 4-aminoan...

Development and Validation of a Spectrophotometric Enzymatic Assay Method for the Quantification of Uric Acid by Using 3 - Hydroxytyramine as a Chromogenic Substrate

Improved uric acid assay in the Paramax.

acid was observed. Analytical recoveries of added etofena. mate and flufenamic acid, respectively, at concentrations of iO, 10-6, and i0 mol/L each exceeded 95%. The detection limit for each was 5 10_8 mol/L. This method can also be used to determine etofenamate and fiufenaniic acid in the synovial liquid. Tissue. We injected, intraperitoneally, 1.48 mg of etofenamate in 1 mL of equal volumes o...

In-situ Enzymatic Generation of Hydrogen Peroxide for Bleaching Purposes

Bleaching detergent formulations contain environmentally unfriendly bleaching agents (perborates and percarbonates), which cause aquatic eutrophication, although without these compounds detergents are much less efficient for the washing processes. In an effort to replace these compounds, in this study, hydrogen peroxide was generated as a bleaching compound by means of enzymatic reactions. Thre...